Naslov
Radical scavenging and antioxidant properties of physiologically active 1, 4-dihydroisonicotinic acid derivatives

Autori
Borović Šunjić, Suzana ; Tirzitis, Gunars ; Tirzite, Dace ; Žarković, Neven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Meeting of the HNE-Club "HNE and Lipid Peroxidation Products" : from basic science to medicine / - Berlin, 2004, P5-P5

Skup
Meeting of the HNE-Club (2 ; 2004)

Mjesto i datum
Berlin, Njemačka, 06.07.2004. - 09.07.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant; 1; 4-dihydroisonicotinic acid derivatives

Sažetak
1, 4-Dihydroisonicotinic acid derivatives (1, 4-DHIND) – a comparatively new class of physiologically active nitrogen heterocycles, possess a wide variety of pharmacological activities and are compounds with very low toxicity, but the mechanism of physiological activity of these compounds is not completely clear. Study was performed to obtain the data of some 1, 4-DHIND radical scavenging and antioxidant activities and their effects on cellular viability. Following compounds were tested: 2, 6-dimethyl-3, 5-diethoxycarbonyl-1, 4-dihydroisonicotinic acid sodium salt ; sodium 2-(2, 6-dimethyl-3, 5-diethoxycarbonyl-1, 4-dihydropyridine-4-carboxamido) glutarate (glutapyron) and sodium 2-(2, 6-dimethyl-3, 5-diethoxycarbonyl-1, 4-dihydropyridine-4-carboxamido) ethane-sulphate (tauropyrone). We have chosen a complex of methods for characterisation of radical scavenging and antioxidative properties of 1, 4-DHIND: N, N-Diphenyl-N’ -picrylhydrazyl (DPPH.) assay ; deoxyribose degradation assay ; ABTS radical cation scavenging assay ; metal ion catalysed peroxidation of phospholipids and ANTIOX-CAP assay. The influence of tested 1, 4-DHIND on cell viability was determined by MTT assay using HeLa cells. The obtained data showed that only 2, 6-dimethyl-3, 5-diethoxycarbonyl-1, 4-dihydroisonicotinic acid possessed radical scavenging and antioxidative activities which could be comparable with the standard antioxidant trolox. Taking together the presented data, 2, 6-dimethyl-3, 5-diethoxycarbonyl-1, 4-dihydroisonicotinic acid seems promising for further antioxidant activities in vivo investigation, while mechanisms of action of other compounds should be further investigated in different direction.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA