Naslov
Ten years of HLA typing at the DNA level in The Tissue Typing Centre - Zagreb

Autori
Žunec, Renata ; Grubić, Zorana ; Čečuk-Jeličić, Esma ; Brkljačić-Šurkalović, Ljerka ; Humar, Ines ; Balog, Vesna ; Palfi, Biserka ; Martinez, Natalija ; Štingl, Katarina ; Kaštelan, Andrija ; Brkljačić-Kerhin, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
2nd scientific symposium with international participation in occasion to 45 years of molecular biology in Croatia and 50 years of double helix : Book of abstracts / - Zagreb, 2003, 51-51

Skup
Scientific symposium with international participation in occasion to 45 years of molecular biology in Croatia and 50 years of double helix

Mjesto i datum
Zagreb, Hrvatska, 20.11.2003. - 21.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Tissue Typing Centre; HLA; DNA; Croatia

Sažetak
The HLA complex of genes on human chromosome 6 encodes proteins that individualise a person's immune system, cause rejection and other immune reactions that can compromise transplanted organs and tissues and are frequently correlated with susceptibility to autoimmune diseases. For over 30 years serology has been the method of choice for HLA tissue typing. When molecular biological methods were introduced in the late 80's of 20th century it became obvious that serological techniques cannot detect all HLA allelic variants whereas it can be achieved by DNA based typing. Since that time, various DNA techniques have been developed for the HLA typing, and most of them are successfully established as routine methods in Tissue Typing Centre. This allowed us to introduce accurate definition of HLA DNA polymorphism in the matching of organs (kidney, heart, liver, pancreas, cornea) as well as for related and unrelated bone marrow transplantation. We have performed numerous disease association studies at the DNA level (ankylosing spondylitis, psoriasis, IDDM, coeliac disease, rheumatoid arthritis, psoriatic arthritis, asthma) which allowed us to find the predisposing or protective HLA genes specific for the Croatian population. As the extensive polymorphism of the HLA genes makes them attractive for human populatian genetics we have also performed DNA based anthropological studies of the entire Croatian population as well as in isolated poputations of Croatian islands (Krk, Hvar). Although a huge amount of work has already been done, further investigations are necessary in order to solve the puzzle named "the biological role of HLA DNA polymorphism".

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA