Naslov
SHORT TANDEM REPEAT LOCI – USEFUL TOOL FOE FOLLOWING CHIMERISM AFTER ALLOGENEIC BONE MARROW TRANSPLANTATION

Autori
Grubić, Zorana ; Štingl, Katarina ; Čečuk-Jeličić, Esma ; Žunec, Renata ; Bogdanić, Vinko ; Labar, Boris ; Kerhin-Brkljačić, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8. hrvatski biološki kongres-Zbornik radova / Besendorfer, Višnja ; Kopjar, Nevenka - Zagreb, 2003, 97-98

Skup
8. hrvatski biološki kongres

Mjesto i datum
Zagreb, Hrvatska, 27.09.2003. - 02.10.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
STR; bone marrow transplantation; chimerism

Sažetak
Mixed chimerism (MC) is a state in which there is a presence of both recipient and donor hemopoietic cells and is frequently observed after bone marrow transplantation (BMT). Previous studies have used cytogenetic techniques, changes in erythrocyte phenotype or RFLP analysis for following chimerism. In cases when none of these markers is useful, analysis of short tandem repeat (STR) loci can be potential diagnostic tool. In this study we monitored 15 patients suffering from various haematological disorders. Twelve of them underwent classical conditioning regimen while the remaining 3 patients were subjected to nonmyeloablative conditioning (minitransplantation). DNA samples were amplified for 5 STR loci (TH01, VWA31, FES/FPS, F13A01, and SE33) and one VNTR locus (D1S80};. In the cases of minitransplantation additional 4 loci (D1S1656, D12S391, D18S535, and D22S683) were also tested. Samples were analysed by electrophoresis in automated ALFexpress sequencer. In all 15 donor recipient-pairs we found differences for at least two STR loci. In most cases the difference was observed for SE33 locus. MC was detected in all 3 cases of minitransplantation, 2 cases with unrelated donor and one ease with related donor. The only ease in which MC was predictive of relapse was the case of a patient who showed full donor chimerism until day 160th post BMT when 40% of recipient cells was detected. In conclusion, this study showed that high informativeness, relative simplicity and short performing time of STR loci analysis makes this method a valuable tool for monitoring chimerism in BMT program.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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