Naslov
The use of primary cell culture in testing effects of growth factor and cytotoxic effects of herbicides

Autori
Radošević, Kristina ; Gaurina Srček, Višnja ; Šimić, Branimir ; Kmetič, Ivana ; Kniewald, Jasna ; Kniewald, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the "3rd Croatian Congress of Toxicology - CROTOX 2004", Plitvice Lakes, Croatia, May 26-29, 2004 / - Zagreb : Hrvatsko toksikološko društvo, 2004

Skup
3rd Croatian Congress of Toxicology - CROTOX 2004

Mjesto i datum
NP Plitvička jezera, Hrvatska, 26.05.2004. - 29.05.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
ovarian primary cell culture; herbicide citotoxicity; growth factor

Sažetak
Proliferation of animal cells in a culture is under control of exogenous growth factors that are mostly present in a serum. The growth factors are more or less specific for particular type of cells and they are polypeptide molecules which act as physiological regulators. It is now considered that they have a substantial role in the development, tissue remodelling, tissue repair and response to injury, haematopoiesis and tumorigenesis. In our recent work, we have found out that the addition of the growth factor isolated from swine brain (BRPP = brain polypeptide), obtained by our own laboratory, has positive influence on the cell proliferation of the cell lines BHK21 C13 and CHO K1 and primary cell culture from rat's ovary. Primary cell culture can be used in the safety assessment of new chemicals, pharmaceuticals and pesticides. Today, practically in each sample of the food or water it is possible to detect very low concentrations of diferrent pesticides, in the amounts usually under the permissible levels, but even these low levels may affect the subtle biochemical processes in organism. Since in vitro methods heve been long used as a basic laboratory tools for defining biological processes, as well as of toxicological implications of xenobiotics in different cellular systems the aim of this work was to see if the growth factor isolated from swine brain (BRPP) has protective effect on cells treated with herbicide lindan. The cytotoxic effect of organochlorine herbicide lindan and protective effect of BRPP was examined on BHK21 C13 and CHO K1 cell lines and ovarian primary cell culture.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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