Naslov
Liječenje teško opečene djece kožnim presadcima uzgojenima in vitro
(Treatment of severely burned children with cultured keratinocyte grafts)

Autori
Vrtar, Zvonimir ; Zekušić, Marija ; Barčot, Zoran ; Prežigalo, Juraj ; Tominac, Mirna ; Kljenak, Anton

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
World Congress of Pediatric Surgery, Book of Abstracts / Barčot, Zoran - Zagreb : Klinika za dječje bolesti, 2004

Skup
World Congress of Pediatric Surgery

Mjesto i datum
Zagreb, Hrvatska, 22.06.2004. - 27.06.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
burns; child; keratinocyte grafts; cell culture

Sažetak
Two girls 1, 5 and 2 years old with extensive burns involving the extremities and the entire back, respectively, were treated with autologus keratinocyte grafts cultured in vitro. The grafts were produced from skin biopsies sized approximately 3 – 4 cm2. Dissolved epithelial cells were cultured as usual on a 3T3 fibroblast layer in appropriate nutrient medium. After three passages, confluent keratinocyte layers were mounted on vaseline gauze, frozen in liquid nitrogen (for the purpose of transportation) and applied on wound beds free of necrotic tissue and infection. The size of the grafts varied from 7 x 10 cm to 10 x 15 cm, and their total areas were 800 cm2 for the first girl and 1200 cm2 for the second one. The mounting tissue was peeled off after four days, leaving small areas and islets of thin epithelium. These foci subsequently coalesced, covering larger areas of the burns. Further recovery was uneventful.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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