Naslov
B- and T-cells in the follicular fluid and peripheral blood of patients undergoing IVF/ET procedures

Autori
Vujisić, Sanja ; Židovec Lepej, Snježana ; Akšamija, Alenka ; Jerković, Lidija ; Sokolić, Božica ; Kupešić, Sanja ; Vince, Adriana

Izvornik
American journal of reproductive immunology (1046-7408) 52 (2004), 6; 379-385

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CD27; B-cells; T-cells; IVF/ET; infertility

Sažetak
PROBLEM: To analyse percentage of total and memory CD27+ B-cells and other lymphocyte subpopulations in the peripheral blood (PB) and follicular fluid (FF) of infertile married couples. METHOD OF STUDY: 48 couples from IVF/ET program were divided into four groups: patients with previous unsuccessful fertilisation (n=13), ectopic pregnancy (n=8), multiple (at least 3) failed IVF/ET (n=18) and missed abortions (n=9). Control group consisted of 15 married couples with healthy children. RESULTS: PB memory CD27+ B-cells were significantly decreased in all groups of infertile patients compared to controls. First group had increased memory B-cells percentages compared to the second group. The differences in the percentages of PB memory B-cells in third and fourth group compared to the first group were not statistically significant. FF memory B-cells in the first and third group were significantly increased compared to second and fourth group. The percentage of total FF B-cells in all groups were significantly decreased compared to their percentage in PB. Male partners of women from the first group had had significantly increased percentages of memory B-cells compared to the partners of women from the second group. Percentage of total T- and B-cells, CD4+ and CD8+ T-cells, NK cells and activated HLA-DR+ T-cells in all groups were not significantly different from controls. We found no statisticaly significant difference between immunoglobuline levels in all groups of patients. We found lower levels of IgA and IgM in follicular fluid compared to serum in all groups. CONCLUSION: Infertile patients have significantly decreased percentage of CD27+ B-cells in the PB. Abnormalities in the memory B-cell compartment may contribute to the pathogenesis of infertility. In the T-cell compartment abnormalities were not detected. It appears that hormonal stimulation did not influence cellular immunity parameters.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA