Naslov
Mitochondrial dysfunction enhances transcription by Gal4 in an Srb10-dependent manner

Autori
Traven, Ana ; Jeličić, Branka ; Filić, Vedrana ; Sopta, Mary

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the FEBS Lecture Course on Cellular Signaling & 4th Dubrovnik Signaling Conference / Đikić, Ivan ; Husnjak, Koraljka - Dubrovnik, 2004, 56-56

Skup
Dubrovnik Signaling Conference (4 ; 2004)

Mjesto i datum
Dubrovnik, Hrvatska, 21.05.2004. - 27.05.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Yeast ; Srb10 ; Gal4 ; mitochondria

Sažetak
Regulation of gene expression by metabolic pathways is a common strategy used by the cell to adjust to its changing energy requirements. One of the best studied examples of such a mechanism is regulation of galactose-inducible genes by the prototypical acidic activator Gal4. In this report we have analyzed the influence of mitochondrial dysfunction on Gal4 activity and show that respiratory deficient cells increase Gal4-dependent transcription. We show that the DNA-binding and activation domains of Gal4 are sufficient for this effect. Furthermore, the cyclin dependent kinase Srb10 (cdk8) is necessary for mitochondrial signaling to Gal4, but this effect is independent of Gal4 S699 phosphorylation. Mitochondrial dysfunction appears to affect regulation of Gal4 binding to DNA. We suggest that Srb10 dependent phosphorylation of a yet unidentified site, potentially in the Gal4 DNA binding domain, or modification of other transcription factors that cooperate with Gal4 in the process of transcriptional activation underlies the effect of Srb10 on Gal4 transcription in response to mitochondrial dysfunction.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

POVEZANOST RADA