Naslov
Sodium and calcium intakes and bone mass in rats revisited

Autori
Šarić, Marija ; Piasek, Martina ; Blanuša, Maja ; Kostial, Krista ; Ilich, Jasminka Z.

Izvornik
Nutrition (0899-9007) 21 (2005), 5; 609-614

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Sodium intake; dietary calcium; urinary calcium; dual energy X-ray absorptiometry (DXA); rats

Sažetak
OBJECTIVE: High sodium intake accompanied by insufficient dietary calcium may have detrimental effects on bone mass. Our study evaluated the effects of both high sodium and decreased calcium intake on bone mineral density (BMD) and bone mineral content (BMC) in rats. METHODS: Four-month-old female Wistar rats were given deionised water or 1.8% solution of sodium chloride in deionised water and fed on normal (1.2%) or marginal (0.33%) calcium in diet during two months. At the end of the experiment BMD and BMC of the whole body and urinary sodium and calcium excretion were evaluated. After that all rats were killed and right femurs removed to assess dry and ash weights. Two way analysis of variance (ANOVA) was used to evaluate effect of salt intake and effect of dietary calcium on above parameters. RESULTS: Salt-loaded animals had greater water consumption during the entire two-month period and significantly lower body weight from week five of the experiment. High salt intake increased urine volume and urinary excretion of both sodium and calcium. Urinary calcium was about five times higher in salt-loaded animals compared to the rats on deionised water irrespective of dietary calcium content. Calcium in diet itself had no significant effect on these parameters. High salt intake slightly but not significantly reduced BMD, BMC and femur weights. Lower calcium in diet significantly reduced BMD and its effect on femur ash weight almost reached level of significance. CONCLUSION: We confirmed benefit of adequate calcium intake to BMD. Under our experimental condition, high salt intake in rats during two months had no statistically significant effect on either femur weights or BMD/BMC even with marginal calcium in diet.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA