Pregled bibliografske jedinice broj: 16080
C-terminal truncation of yeast SerRS is toxic for Saccharomyces cerevisiae due to altered mechanism of substrate recognition
C-terminal truncation of yeast SerRS is toxic for Saccharomyces cerevisiae due to altered mechanism of substrate recognition // FEBS letters, 439 (1998), 3; 235-240 doi:10.1016/S0014-5793(98)01376-3 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 16080 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
C-terminal truncation of yeast SerRS is toxic for
Saccharomyces cerevisiae due to altered mechanism
of substrate recognition
Autori
Lenhard, Boris ; Praetoruis-Ibba, Mette ; Filipić, Sanda ; Soll, Dieter ; Weygand-Đurašević, Ivana
Izvornik
FEBS letters (0014-5793) 439
(1998), 3;
235-240
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
aminoacyl-tRNA synthetases ; tRNA recognition ; toxicity ; tRNA-dependent amino acid recognition ; protein degradation
Sažetak
Like all other eukaryal cytosolic seryl-tRNA synthetase (SerRS) enzymes, Saccharomyces cerevisiae SerRS contains a C-terminal extension not found in the enzymes of eubacterial and archaeal origin. Overexpression of C-terminally truncated SerRS lacking the 20-amino acid appended domain (SerRSC20), is toxic to S. cerevisiae possibly because of altered substrate recognition. Compared to wild-type SerRS the truncated enzyme displays impaired tRNA-dependent serine recognition and is less stable. This suggests that the C-terminal peptide is important for the formation or maintenance of the enzyme structure optimal for substrate binding and catalysis.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
119411
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
