Naslov
Expression of CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase) on CD8+ T-lymphocytes of persons infected with Human Immunodefficiency Virus type 1

Autori
Židovec Lepej, Snježana ; Begovac, Josip ; Vince, Adriana ; Brkić, Lada ; Remenar, Anica ; Kniewald, Tihana

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), ostalo

Izvornik
3rd Croatian Scientific Conference about Biotechnology and Food / - Zagreb : Hrvatsko Društvo za Biotehnologiju ; Medicinska naklada, 2003, 309-17

Skup
3rd Croatian Scientific Conference about Biotechnology and Food

Mjesto i datum
Zagreb, Hrvatska, 17.02.2003. - 20.02.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
CD38; HIV-1; Quantitative flow cytometry

Sažetak
Objective: Our goal was to analyse the expression of the activation marker CD38 on CD8+ T-lymphocytes of HIV-infected persons. We also investigated the influence of highly active antiretroviral therapy (HAART) on the expression of CD38. Methods: The study enrolled 25 treatment-naive patients with advanced HIV-disease with high plasma viremia (median 4.8 log10 copies of HIV-1 RNA/ml) and low absolute counts of CD4+ T-lymphocytes (median 98 cells/&micro ; ; ; l). Treatment regiment consisted of zidovudine or stavudine, lamivudine and indinavir at standard doses. Plasma viremia was quantified by RT-PCR and lymphocyte subpopulations were determined by flow cytometry. CD38 expression was quantified by using CELLQUANT kit (Biocytex, France). Results: Untreated symptomatic HIV-patients had significantly higher percentages of CD38+CD8+ T-lymphocytes (median 46, 3%) compared to healthy controls (median 13, 4%, p<0, 001). HAART significantly reduced plasma viremia to undetectable levels after the third month of treatment (median 1.69 log of HIV-1 RNA copies/ml) in 82% of patients. Over the next 11 months, undetectable plasma viremia was achieved in 93% of patients. HAART-induced decrease in viral load was parallel to the significant decrease in the percentages of CD38+CD8+ T-lymphocytes. Percentages of CD38+CD8+ T-lymphocytes in 41% of patients returned to normal values after 12 months of treatment. However, numbers of CD38 molecules expressed on bright CD8+ T-lymphocytes of patients with at least 9 months of undetectable viremia were significantly increased (median 5219 anti-CD38 antibody binding sites) compared to healthy controls (median 947 anti-CD38 antibody binding sites). Absolute counts of CD4+ T-lymphocytes significantly increased after the first month of HAART (median 203 cells/&micro ; ; ; l) and reached a median of 215 cells/&micro ; ; ; l after 12 months. Conclusions: Infection with HIV-1 increased the expression of activation marker CD38 on CD8+ T-lymphocytes. Increased numbers of CD38 molecules on CD8+ T-lymphocytes after 11 months of HAART-induced undetectable plasma viremia suggest residual viral activity.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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