Naslov
Electrospray Ionization Fourier Transform Ion Cyclotron Resonance at 9.4 T

Autori
Senko, Michael W. ; Hendrickson, Christopher L. ; Paša-Tolić, Ljiljana ; Marto, Jarrod A. ; White, Forest M. ; Guan, Shenheng ; Marshall, Alan G.

Izvornik
RCM. Rapid communications in mass spectrometry (0951-4198) 10 (1996), 14; 1824-1828

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
tandem mass-spectrometry ; large biomolecules ; large molecules ; isotopic distributions ; carbonic-anhydrase ; charged ions ; spectra ; proteins ; cell ; remeasurement

Sažetak
We present the first results from a new electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer operated at a magnetic field of 9.4 T (i.e. greater than or equal to 2.4 T higher than for any prior FTICR instrument). The 9.4 T instrument provides substantially Improved performance for large molecules (greater than or equal to 50% increase in mass resolving power) and complex mixtures (greater than or equal to 100% increase in dynamic range) compared to lower-field (less than or equal to 6 T) instruments, The higher magnetic field makes possible larger trapped-ion population without introduction of significant space-charge effects such as spectral peak shift and/or distortion, and coalescence of closely-spaced resonances, For bovine ubiquitin (8.6 kDa) we observe accurate relative isotopic abundances at a signal-to-noise ratio greater than 1000:1, whereas a complete nozzle-skimmer dissociation electrospray ionization (ESI) FTICR mass spectrum of bovine carbonic anhydrase (29 kDa) is achieved from a single scan with a signal-to-noise ratio of more than 250:1, Finally, we are able to obtain mass resolving power, m/Delta m >200 000, routinely for porcine serum albumin (67 kDa). The present performance guides further modifications of the instrument, which should lead to significant further improvements.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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