Naslov
Early pregnancy decidual lymphocytes lyse NK resistant targets by perforin and Fas/FasL cytolytic mechanisms

Autori
Bogovic Crnčić, Tatjana ; Štrbo, Nataša ; Laškarin, Gordana ; Sotošek Tokmadžić, Vlatka ; Ćupurdija, Kristijan ; Juretić, Koraljka ; Dorčić, Dorotea ; Dupor, Jana ; Sršen, Suzana ; Vlastelić, Ivica ; Randić, Ljiljana ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EFIS symposium and postgraduate course : Molecular and cellular interactions in chronic inflammatory autoimmune diseases : Abstract book / Mostarica-Stojković, Marija - Beograd, 2004

Skup
EFIS symposium and postgraduate course : Molecular and cellular interactions in chronic inflammatory autoimmune diseases

Mjesto i datum
Crna Gora; Beograd, Srbija, 29.08.2004. - 02.09.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pregnancy; decidua; lymphocytes; Th1 cytokines; perforin; FasL

Sažetak
The prominent feature of early pregnancy decidua is infiltration of CD3-CD16-CD56bright+ NK (dNK) cells followed by smaller number of macrophages and T cells. dNK are activated and filled with cytolytic mediators. Their activity is regulated by complex cytokine network. We analyzed cytotoxicity of decidual and peripheral blood lymphocytes (DL and PBL) against NK resistant targets under influence of Th1 cytokines. Decidual tissue and peripheral blood cells were obtained from elective termination of normal human pregnancies (6-10 weeks). Decidual tissue was enzymatically digested. Decidual and peripheral blood mononuclears were obtained by density gradient centrifugation. DL and PBL were collected following 18 or/and 72 hrs culture in the medium or in the presence of Th1 cytokines. Cytotoxicity assay, direct and indirect immunofluorescency were analyzed by flow cytometry. Non-stimulated PBL did not lyse NK resistant P815 and P815Fas targets while PBL stimulated with Th1 cytokines lysed more P815Fas than P815 cells. Non-stimulated DL lysed K562, but also P815 and P815Fas cells and this cytolytic effect could not be increased by Th1 cytokine stimulation. Non-stimulated and stimulated DL had higher cytotoxicity against P815Fas than P815 cells. 7% of CD56bright+ cells expressed FasL following IL-15 stimulation. DL cultured in the medium are activated and able to lyse NK resistant targets by perforin and FasL cytolytic mediators.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA