Naslov
Identification of HIV-1 protease cleavage site in human C1-inhibitor

Autori
Gerenčer, Damir ; Burek, Vitomir

Izvornik
Virus research (0168-1702) 105 (2004); 97-100

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HIV-1 protease; Limited proteolysis; Complement proteins; Protease cleavage site; HIV-1 pathogenesis

Sažetak
We have investigated the ability of HIV-1 protease to cleave human complement proteins of the slassical complement pathway: Ciq, C2 and C4 as well as regulatory protein, C1-inhibitor. Purified complement proteins were incubated with recombinant HIV-1 protease in vitro and analysed by SDS-PAGE and immunoblotting assay. The only cleavage site was found in N-terminal region of C-1inhibitor, and it was located between residues Leu-32 as determined by amino acid sequence analysis of the 85kDa proteolytic fragment after 12 Edman degradation cycles. The HIV-1 protease cleavage site were not found in C1q, C2 and C4 protein. HIV-1 protease-susceptible site in N-terminal region of C-1 inhibitor is very close to the cleavage sites of some other proteases that are able to induce N-terminal proteolysis of the protein

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA