Naslov
Identification and analysis of cDNAs encoding two nucleoside diphosphate kinases (NDPK/Nm23) from the marine sponge Suberites domuncula

Autori
Harcet, Matija ; Lukić-Bilela, Lada ; Ćetković, Helena ; Müller, E.G. Werner ; Gamulin, Vera

Izvornik
Croatica chemica acta (0011-1643) 78 (2005), 3; 343-348

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antimetastatic proteins; nucleoside diphosphate kinases; sponge cDNA library; Porifera

Sažetak
Suberites domuncula is a member of the most ancient and simplest extant phylum of multicellular animals ; Sponges (Porifera). Database of S. domucula expressed sequence tags (ESTs) was recently constructed by random cDNA sequencing. Two NDPK/Nm23 proteins from the sponge Suberites domuncula are reported here. Sponge proteins were named Nm23-SD1 and Nm23-SD6, because they display the highest sequence similarity with human Nm23-H1 and -H6 proteins. Overall sequence conservation of Nm23-SD1 with human Nm23-H1 is very high - 79% (71% identity). Nm23-SD6 possesses an insertion at the C-terminus and displays 55% overall homology (40% identity) with human Nm23-H6. Secondary structure predictions for both sponge and human Nm23 protein pairs are almost identical. S. domuncula Nm23 proteins display high similarity with homologues from mammals/human, higher than with i.e. NDPK/Nm23 proteins from Drosophila or other invertebrates. Sponge Nm23 proteins are more similar to mammalian/human Nm23 proteins than most known Nm23 proteins from invertebrates.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA