Naslov
Cytokines and cytolytic mechanisms at the maternal-fetal interface, Pozvano predavanje na: 3rd International Postgraduate School of Immunogenetics in Transfusion Medicine, Clinical immunogenetics and cell therapy, Ljubljana, 06.-09.06, 2004.

Autori
Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Cytokines and cytolytic mechanisms at the maternal-fetal interface. 3rd International Postgraduate School of Immunogenetics in Transfusion Medicine, Clinical immunogenetics and cell therapy

Mjesto i datum
Ljubljana, Slovenija, 06.06.2004. - 09.06.2004

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Natural killer (NK) cells; Macrophages; Dendritic cells and T cells; Pregnancy

Sažetak
During normal pregnancy tissue of fetal origin-trophoblast develops and differentiates within uterus in the close contact with maternal tissue. Maternal decidua harbors immunocompetent CD45+ cells such as natural killer (NK) cells, macrophages, dendritic cells and T cells. Decidual NK cells are predominant cell population with a phenotype CD56++CD16-. These cells show characteristics of activated cells and are equipped with high cytolytic potential (Perforin, Granzymes, FasL) and might perform an immune surveillance function protecting both mother and fetus. There is a general opinion that IL-2 is not expressed at the maternal-fetal interface during normal pregnancy. However, recent results by ours and others clearly showed that IL-15 Th1 type cytokine which shows many similarities with IL-2 is expressed at the maternal-fetal interface. IL-15 can affect cytolytic activity of maternal lymphocytes which infiltrate decidua during first trimester pregnancy. IL-18 a recently discovered Th1 type cytokine is also expressed at the maternal-fetal interface. Accordingly, decidual T and NK cells can be activated by Th1 type cytokines and theoretically can gain the potential of lymphokine-activated killer cells (LAK). LAK cells express stronger cytolytic activity to different NK sensitive and NK resistant cell lines as a result of up-regulation of cytolytic mediators in these cells. Perforin and FasL are the main mediators in acute cytolytic pathway. In the broad panels of experiments the aim was to examine a) expression of Th1 type cytokines IL-15 and IL-18 at the interface, and b) decidual lymphocytes cytotoxic activity against NK sensitive and NK resistant but LAK sensitive cell lines to obtain an insight to the level of their activation. Simultaneously peripheral blood lymphocytes cytolytic activity was investigated. Main results will be presented and discussed. The results clearly showed that DL use both perforin and FasL-Fas pathways at the maternal-fetal interface.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA