Naslov
Novi pristupi u analizi imunoloških mehanizama u infektivnim bolestima
(New approaches to the analysis of immunological mechanisms in infectious diseases)

Autori
Židovec Lepej, Snježana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
4th Croatian congress on infectious diseases with international participation Abstract book / - Zagreb, 2004, 61-61

Skup
4th Croatian congress on infectious diseases with international participation

Mjesto i datum
Opatija, Hrvatska, 02.10.2004. - 06.10.2004

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
imunološke tehnike infektivne bolesti citokini tetrameri
(immunological techniques infectious diseases cytokines tetramers)

Sažetak
New approaches to the analysis of immunological mechanisms in infectious diseases S. Židovec Lepej University Hospital for Infectious Diseases “ Dr. Fran Mihaljević” , Zagreb, Croatia Powerful new immunological techniques, combined with many of the traditional assays for measuring immune responses, are rapidly enabling a better understanding of the complexity of immunity to infectious agents. Lymphocyte responses in infectious diseases are no longer limited to phenotype analysis but also include functional assays (intracellular cytokine synthesis, antigen-specific immunity etc.). Cytokine analysis in infectious diseases can be achieved by a broad variety of assays including: (1) bioassays and immunoassays for soluble cytokines and cytokine receptors, (2) intracellular cytokine synthesis analysis by flow cytometry, (3) quantification of cytokine or cytokine receptors transcripts by real time quantitative RT-PCR or RNase protection assays, (4) detection of cytokines in tissues by immunofluorescence and immunocytochemistry and (5) cytokine profiling by proteomics and genomics. The advent of technologies enabling the detection and quantification of antigen-specific T-cell responses (tetramers, intracellular cytokine staining and enzyme-linked immunospot assays) has improved the analysis of the antiviral immunity in different models (particularly HIV, EBV, CMV). Accurate measurement of antigen-specific responses is particularly important in evaluating vaccine efficacy. New techniques for the assessment of immune responses have had a significant impact on biological and biomedical research of infectious diseases. Possible implementation of these techniques in routine diagnostic work-up will add a new dimension to clinical diagnostic medicine.

Izvorni jezik
Engleski

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