Naslov
TLC analysis of amino acids and flavonoids in Cerastium grandiflorum Waldst. & Kit.

Autori
Bilušić Vundać, Vjera ; Maleš, Željan ; Plazibat, Miško.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Šegudović N. - Zagreb : ITG d.o.o., Zagreb, 2004, 54-54

Skup
10th International Symposium on Separation Sciences-New achievements in chromatography

Mjesto i datum
Opatija, Hrvatska, 12.10.2004. - 15.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cerastium grandiflorum ; amino acids ; flavonoids ; TLC

Sažetak
Cerastium grandiflorum Waldst. & Kit. (= Cerastium nodosum Buschm.) is an endemic species of Dinaric Karst belonging to the family Caryophyllaceae. That is a white to greyish-tomentose perennial which grows in sods on dry rocky places. Leaves are narrowly linear, with pointy apices. The inflorescence consists of 7-15 flowers which form a fastigiate cyme. Calyx is formed by 6-8 mm long, nearly subobtuse sepals. Corolla consists of 5 white petals which are almost three times longer than the sepals, this explains the Latin term “ grandiflorum” in the plant name. There are ten stamens, and one pistil with tomentose ovary. Fruit is straight capsule with many seeds which are flat and black. The flowering time is from May to July according to altitude and exposure. The plant material consisted of aerial parts of Cerastium grandiflorum which were collected during August 2002 and June 2003 on Mts. Mosor (sample 1) and Velebit (sample 2). The presence of amino acids by thin-layer chromatography was performed on cellulose F, in two mobile phases: n-butanol-acetone-acetic acid-water (35:35:10:20 V/V/V/V) and n-butanol-acetic acid-water. The detection of separated amino acids was carried out by ninhidrin reagent. The presence of flavonoids was determined by thin-layer chromatography on silica gel 60 F 254 in two mobile phases: ethyl acetate-formic acid-acetic acid-water (100:11:11:27 V/V/V/V) and ethyl acetate-formic acid-water (8:1:1 V/V/V). The detection of separated flavonoids was performed after spraying the chromatograms with NST/PEG reagent and looking in UV light at 365 nm. The aerial parts of sample 1 showed the presence of isoleucine, tryptophan,  -aminobutyric acid, proline, alanine, threonine, glutamine, arginine and ornithine. The same amino acids were proved in sample 2, along with amino acid tyrosine. Both samples showed the presence of flavonoid glycoside hyperoside and caffeic acid, along with flavonoid aglycone quercetin

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA