Naslov
Physiologic and pathogenetic significance of perforin in pregnancy

Autori
Rukavina, Daniel ; Laškarin, Gordana ; Štrbo, Nataša ; Sotošek, Vlatka ; Podack, Eckhard R.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the VII International Congress of Reproductive Immunology ; u: American Journal of Reproductive Immunology / Gleicher, Norbert - Kopenhagen : Munksgaard, 1998, 250-250

Skup
International Congress of Reproductive Immunology (7 ; 1998)

Mjesto i datum
New Delhi, Indija, 27.10.1998. - 30.10.1998

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
perforin; pregnancy; physiology; pathophisology

Sažetak
Perforin (P) positive lymphocytes are present in high concentration in the metrial gland of rodents' pregnancy and first trimester human pregnancy decidua. Experiments with P deficient mice showed that P plays a major role in CTL and NK cell-mediated cytotoxicity, but is not necessary for normal pregnancy. However, mice simultaneously deficient for both cytolytic pathways (Fas L/Fas and Perforin double deficient mice-DD) are short lived and female mice are infertile. Mice homozygous for Fas-L defect, but heterozygous at the P locus and expressing one functional P allele are fertile and protected from early death. The analyses of P expression in peripheral blood lymphocytes (PBL) of nonpregnant (NP) first trimester pregnancy (FTP) and term pregnancy (TP) females showed considerable differences: 1) Proportion of P+ cells increased significantly at the end of the pregnancy compared to FTP (22% vs. 37%) ; 2) In the FTP 18% of P+ cells are CD4+ which is 3 times higher than in NP ; 3) drastic decrease of CD16+ cells in the population of P+ cells (from 45% in NP to 4% to FTP), and increase at TP (67%). In the FTP decidua CD3 CD56 bright+CD16    decidual lymphocytes (DL) are Pbright+, which was confirmed by analyzing CD3 clones. In pathological pregnancies (anembryonic pregnancy and missed abortion) both the percentage of CD4+ cells among P+ cells and the content of P/cell were 3 times lower compared to NP. Decidual adherent cells (DAC) and/or their supernatants have pivotal role in upregulation of P expression in decidual lymphocytes. These effects can be blocked by anti IL-15 antibodies. In our opinion direct and indirect (cytokine and hormone mediated) interactions between DAC and decidual lymphocytes is responsible for high level of P expression in DL.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA