Naslov
Serotonin transporter kinetics in rats selected for extreme values of platelet serotonin level

Autori
Čičin-Šain, Lipa ; Froebe, Ana ; Bordukalo-Nikšić, Tatjana ; Jernej, Branimir

Izvornik
Life sciences (0024-3205) 77 (2005), 4; 452-461

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
platelet serotonin; serotonin transporter; selective breeding; rat platelets; transporter kinetics

Sažetak
By selective breeding of Wistar rats for the extreme values of platelet serotonin (5HT) level (PSL), we have developed earlier two sublines of animals differing markedly in this parameter. Further studies, performed on the protein and mRNA levels, revealed platelet serotonin transporter (5HTt) as parameter underlying mentioned differences in PSL between sublines. In this work, we have performed full-kinetic analysis of platelet serotonin uptake (PSU) in animals from the genetically selected sublines. The results demonstrated marked differences in maximal velocity (Vmax) of the 5HT transporter, as contrasted to the lack of any difference in the Michaelis constant (KM). High correlation between PSL and Vmax of PSU was demonstrated, revealing that the number of membrane 5HT transporter sites is under genetic control and responsible for marked differences in PSL between high- and low-5HT sublines. These results enabled further selective breeeding of animals for the extremes of Vmax of platelet 5HT transporter, and so the development of more specific model "Wistar-Zagreb 5HT rats".

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA