Pregled bibliografske jedinice broj: 157742
TLC analysis of free amino acids in Althaeae radix, treated with different γ -irradiation doses
TLC analysis of free amino acids in Althaeae radix, treated with different γ -irradiation doses // 51st Annual Congress of the Society for Medicinal Plant Research – ; Book of Abstracts / Blaschek W. , Alban S. (ur.).
Kiehl, 2003. (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
TLC analysis of free amino acids in Althaeae radix, treated with different γ -irradiation doses
Autori
Gašpar Randić, Zita ; Maleš, Željan ; Vestermajer, Goran.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
51st Annual Congress of the Society for Medicinal Plant Research – ; Book of Abstracts
/ Blaschek W. , Alban S. - Kiehl, 2003
Skup
51st Annual Congress of the Society for Medicinal Plant Research
Mjesto i datum
Kiel, Njemačka, 31.08.2003. - 04.09.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Althaeae radix; γ – irradiation; amino acids; TLC
Sažetak
Althaeae radix (obtained from Althaea officinalis L., Malvaceae) contains mucilages (about 25-35%) with galacturonic acid, glucuronic acid and rhamnose as major components. Due to such a favourable composition with a lot of mucilage and starch, the herbal drug is often subject to microbiological contamination. γ – irradiation is a contemporary effective, economic, fast, practical and commercial method for a microbial decontamination, which cannot be used with such herbal drugs abundant with mucilage, since they will be biodegraded. This study aimed to find out what happens to the free amino acids in this drug with much mucilage, at different γ – irradiation doses of 1, 5 and 10 kGy. These doses are most frequently used for a microbial decontamination. By a TLC method it has been discovered that in Althaeae radix the following free amino acids appear: asparagine (Asn), lysine (Lys), alanine (Ala), γ – aminobutyric acid (Gaba) as well as some other amino acids in smaller quantities. TLC method has been performed on cellulose, using solvent systems: n-butanol – acetone – glacial acetic acid – water (35:35:10:20 V/V/V/V) and n-butanol – glacial acetic acid – water (50:10:40 V/V/V). Detection has been carried out by a ninhydrin reagent. The TLC method proved that the tested Althaeae radix samples contain identical free amino acids irrespective of the γ – irradiation treatment.
Izvorni jezik
Engleski
Znanstvena područja
Farmacija
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