Naslov
Sensitivity of B-cell chronic lymphocytic leukemia to rituximab and campath 1H and correlation with the expression of cell cycle regulatory proteins

Autori
Grdiša, Mira

Izvornik
Croatian medical journal (0353-9504) 45 (2004), 2; 136-141

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
anti-CD20 (Rituximab); anti-CD52 (Campath 1H); B-CLL; apoptosis; cell cycle; regulatory proteins; p27; cyclins

Sažetak
Aim: Influence of monoclonal antibodies anti-CD20 (Rituximab) and anti-CD52 (Campath1H) on viability of B-cells from chronic lymphocytic leukemia (B-CLL) patients, in comparison with Fludarabine (Fluda) was examined. The effect of these agents on the expression of cell cycle regulatory proteins in vitro was also investigated. Methods: B-CLL cells were incubated in vitro in the presence of Rituximab, Campath 1H and Fludarabine (cytotoxic drug). Viability of the cells was measured by MTT test (3-(4, 5-dimethylthiazol-2-yl)-2, 5-dyphenyltetrazolium bromide). Gel electrophoresis and Western blotting were used to determine the influence of these agents on the expression of cell cycle regulatory proteins. Results: In comparison with Fluda, both antibodies were less toxic to B-CLL cells. Combination of Campath 1H or Rituximab with Fluda did not have a better effect than Fluda alone. Both antibodies decreased the level of p27 protein and increased the level of p23, similarly as Fluda. The levels of cyc D3 and cyc E expression had no remarkable change. The level of cyc D2 was somewhat increased in the presence of Campath 1H, but in the presence of Rituximab it decreased slightly or did not change. Treatment of B-CLL cells with Fluda alone induced significant decrease of cyc D2. Conclusion: These results demonstrated that Campath 1H and Rituximab, as well as Fluda have cytotoxic effect on B-CLL cells. They most likely induced apoptosis of B-CLL cells, but their activity is mediated through different pathways.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA