Naslov
Satellite sequences in pericentromeric heterochromatin of species from the genus Palorus (Insecta, Coleoptera) - evidence for the library hypothesis

Autori
Meštrović, Nevenka ; Ugarković, Đurđica ; Mravinac, Brankica ; Plohl, Miroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytogenetics and Cell Genetics (13th International Chromosome Conference) / Klinger, H.P. - Basel : Karger Publishers, 1998, 132-132

Skup
13th International Chromosome Conference

Mjesto i datum
Ancona, Italija, 08.09.1998. - 12.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
satellite DNA; evolution

Sažetak
Insect species belonging to the family Tenebrionidae (Coleoptera), have a significant genomic fraction (20-40%) composed of tandemly repeated sequences (satellite DNAs) located in pericentromeric heterochromatin of all chromosomes (Petitpierre et al. 1995). Within the genus Palorus, each of analyzed species (P. ratzeburgii, P. subdepressus, P. ficicola, P. genalis and P. cerylonoides) has its own species-specific, highly abundant, major satellite DNA. Using PCR assay it has been shown that all sequences are present in each of the Palorus species: besides a major satellite, the others are in the form of low-copy-number repeats. Underrepresented repeats appeared to be extremely conserved with respect to the corresponding satellite DNA, and organized in tandem despite their low genomic abundance of up to only few hundred copies. Low-copy-number satellites are dispersed between the large arrays of the major satellite in the regions of pericentromeric heterochromatin of all chromosomes. Our results confirm the hypothesis that related species share a "library" of conserved satellite sequences, some of which could be amplified into a major satellite (Fry and Salser 1977). Quantitative changes in satellite DNAs, could possibly occur in the course of speciation process, thus forming species specific profile of satellite DNAs. References Fry F, Salser W: Nucleotide sequences of HS-a satellite DNA from kangaroo rat Dipodomys ordii and characterization of similar sequences in other rodents. Cell 1977 12:1069-1074. Petitpierre E, Juan C, Pons J, Plohl M and Ugarković D: (1995). Satellite DNA and constitutive heterochromatin in tenebrionid beetles. In: PE Brandham and M Bennett, eds. Kew Chromosome Conference IV. Royal Botanic Gardens , Kew, pp351-362.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA