Pregled bibliografske jedinice broj: 156348
Tankoslojna kromatografija slobodnih aminokiselina korijena bijelog sljeza (Althaeae radix), izloženog različitim dozama gama zračenja
Tankoslojna kromatografija slobodnih aminokiselina korijena bijelog sljeza (Althaeae radix), izloženog različitim dozama gama zračenja // Farmaceutski glasnik : glasilo Hrvatskog farmaceutskog društva, 60 (2004), 1; 1-6 (međunarodna recenzija, članak, znanstveni)
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Naslov
Tankoslojna kromatografija slobodnih aminokiselina korijena bijelog sljeza (Althaeae radix), izloženog različitim dozama gama zračenja
(TLC analysis of free amino acids in Althaeae radix, treated with different γ -irradiation doses)
Autori
Gašpar Randić, Zita ; Maleš, Željan ; Vestermajer, Goran
Izvornik
Farmaceutski glasnik : glasilo Hrvatskog farmaceutskog društva (0014-8202) 60
(2004), 1;
1-6
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Althaeae radix) ; gama zračenje ; tankoslojna kromatografija ; aminokiselina
(Althaeae radix ; γ -irradiation ; amino acids ; TLC)
Sažetak
Althaeae radix (obtained from Althaea officinalis L., Malvaceae) contains mucilages (about 25-35%) with galacturonic acid, glucuronic acid and rhamnose as major components. Due to the specific composition with a lot of mucilage and starch, such herbal drugs are often a subject of microbiological contamination. Although γ -irradiation is a contemporary effective, economic, fast, practical and commercial method for a microbial decontamination, it cannot be applied when herbal drugs are abundant with mucilage, since these substances will be degraded. Our study aimed to find out what happened to the free amino acids when this drug was exposed to γ -irradiation doses of 1, 5 and 10 kGy. These doses are most frequently used for a microbial decontamination. By a TLC method it has been discovered that Althaeae radix contained free amino acids as follows: γ -aminobutyric acid (Gaba), alanine (Ala), arginine (Arg), asparagine (Asn), lysine (Lys), as well as some other amino acids in smaller quantities. TLC method has been performed on cellulose and silica gel, using solvent system: n-butanol - acetone - glacial acetic acid - water (35:35:10:20 V/V/V/V). Detection has been carried out by a ninhydrin reagent. The TLC method proved that the tested samples of Althaeae radix contained identical amino acids pattern irrespective of the γ -irradiation treatment.
Izvorni jezik
Hrvatski
Znanstvena područja
Farmacija
POVEZANOST RADA
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Časopis indeksira:
- Scopus
Uključenost u ostale bibliografske baze podataka::
- Excerpta Medica
