Naslov
Restrikcijska naliza kloroplastne DNA triju vrsta roda Thymus (Lamiaceae)

Autori
Hazler Pilepić, Kroata ; Lovrinov, Miran ; Plazibat, Miško ; Maleš, Željan ; Bilušić Vundać, Vjera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka = Book of abstracts / Mitić, Božena ; Šoštarić, Renata - Zagreb : Hrvatsko Botaničko Društvo, 2004, 129-130

Skup
Prvi hrvatski botanički simpozij [s međunarodnim sudjelovanjem = 1st Croatian Botanical Symposium [with International Participation

Mjesto i datum
Zagreb, Hrvatska, 30.09.2004. - 02.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Thymus spp. ; Kloroplastna DNA ; restrikcijska analiza
(Thymus spp. ; chloroplast DNA ; restriction analysis)

Sažetak
Preliminary investigations of cpDNA polymorphism have been performed on individuals of species T. serpyllum L., T. vulgaris L. and T. bracteosus Vis. ex Benth. Members of the genus Thymus, as well as numerous species belonging to the family Lamiaceae, are of interest due to presence of essential oils and their use in pharmacy, cosmetics and nutrition. Numerous investigations showe high degree of morphological and chemical variability among species of genus Thymus. For example, T. serpyllum L. are morphologically very variable while T. vulgaris L. has a large number of chemotypes. Chemical composition and anatomical characteristics of the T. bracteosus Vis. ex Benth., an endemic species of Dinaric Karst, is currently in investigation. With aim to test cpDNA variability among Thymus species as well as suitability of the cpDNA analyses for intra and interspecific phylogenetic study of genus Thymus, preliminary investigations of cpDNA polymorphism have been performed on individuals of species T. serpyllum L., T. vulgaris L. and T. bracteosus Vis. ex Benth. Six regions in large singe copy of chloroplast DNA have been amplified by PCR with universal chloroplast primers. Regions obtained are between genes psaA and trnS, trnC and trnD, psbC and trnS, trnF and trnV1, trnH and trnK1r and finally trnK1 and trnK2r. Six fragment obtained are same in size for all species. Amplification products were digested using 4-base recognition endonucleases HinfI and TaqI and restriction patterns obtained were compared. T. serpyllum L. differs from the other species in both restriction profiles of region psaA – trnS. Intraspecific polymorphism was detected among individuals of T. vulgaris L. from different geographic locations in regions trnC - trnD, trnF - trnV1 i trnK1 - trnK2r. Obtained results point out the high conservation of chloroplast genome among compared species. Also presence of the geographic haplotypes in this genus are possible.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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