Naslov
On the role of T lymphocytes in stimulation of humoral immunity induced by peptidoglycan-monomer linked with zinc

Autori
Ravlić Gulan, Jagoda ; Radošević-Stašić, Biserka ; Trobonjača, Zlatko ; Petković, Marija ; Ćuk, Mira ; Rukavina, Daniel

Izvornik
International archives of allergy and immunology (1018-2438) 119 (1999), 1; 13-22

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Peptidoglycan monomer linked with zinc; humoral immunity; SRBC; immunoglobulins; plaque forming cells; spleen; bone marrow; thymus; FACS analysis

Sažetak
Effects of peptidoglycan linked with zinc (PGM-Zn) were investigated on PFC-generation to SRBC and SRBC-unrelated antibody production in primary and secondary immune response in mice depleted in vivo of CD4+ and/or CD8+ T lymphocytes. PGM-Zn in non-depleted mice stimulated the PFC generation and IgM or IgG and IgG1 production in primary and secondary reaction. Single depletion of CD4 or CD8+ T cells did not change this ability. The effects of PGM-Zn after CD8+ depletion were even greater than those in non-depleted mice. Depletion of both T cell subsets, however, completely abrogated immunostimulatory effects of PGM on PFC generation (primary and secondary response), as well as, on primary SRBC-unrelated antibody production, leaving unchanged only the increase of IgG in secondary response. Immunostimulatory effects and isotype switching to IgG1 and IgG2a correlated with the changes in splenic CD4+, CD8+, CD5+ cells, pointing to the regulatory role of these cells and/or their cytokines in PGM-Zn induced immunostimulation. Altogether the data suggest that PGM-Zn may potentiate the co-stimulatory signals coming from activated T cells and act on B cells without the T-cell help.

Izvorni jezik
Engleski

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