Naslov
Serum dipeptidyl peptidase IV (DPPIV/CD 26) and alkaline phosphatase activities in children with Crohn's disease

Autori
Detel, Dijana ; Peršić, Mladen ; Lupis, Tamara ; Varljen, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EJB, The FEBS Journal / - : Wiley-Blackwell, 2004, 106-106, volume 271, supplement 1

Skup
29th Meeting of the Federation of the European Biochemical Societies

Mjesto i datum
Varšava, Poljska, 26.06.2004. - 01.07.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dipeptidyl peptidase IV (DPPIV/CD 26); alkaline phosphatase; Crohn's disease

Sažetak
The serin protease dipeptidyl peptidase IV (DPPIV/CD 26) is a widely distributed type II plasma membrane glycoprotein. It is expressed both as a soluble form in plasma and on the surface of nearly all mammalian tissues. By virtue of its exopeptidase activity DPPIV is involved in the regulation of the systemic immune response to local inflammatory reaction. Crohn´s disease (CD) and colitis ulcerosa (UC) are chronic gastrointestinal inflammatory bowel disease (IBD). The exact cause of IBD remains unknown but current data suggest that the immune dysbalance in genetically susceptible individuals is an important factor in the pathogenesis of IBD. The aim of this study was to establish the serum DPPIV and alkaline phosphatase (ALP) activities in children with active IBD, compared to the levels found in healthy control group. The diagnosis of CD was established by using the usual clinical, laboratory, endoscopic and histological criteria. CD activity was evaluated by using the Paediatric Crohn´s Disease Activity Index [PCDAI]. The study was performed on 15 children with active CD ([PCDAI]>35). The control group included 40 healthy children (HC). Both groups were not statistically different regarding age (median range 15&plusmn ; ; 2 years). The determination of DPPIV activity in serum was performed using Gly-Pro-p-nitroanilide as a substrate. Serum DPPIV activity in children with active CD was significantly decreased compared with the levels found in healthy control (61, 80&plusmn ; ; 14, 37 U/L versus 94, 33&plusmn ; ; 25, 38 U/L in HC, p<0, 05). The DPPIV activity in CD patients was inversely correlated with [PCDAI] score. Plasma levels of ALP were not significantly different between the two analysed groups. The results of this study suggest that the decreased serum DPPIV activity in CD patients can be connected with the adaptive systemic immune response to an intestinal damage and/or local inflammatory reaction. Soluble DPPIV may be involved in the pathophysiology of IBD, so it appears to be useful as a new disease activity index in the diagnosis of Crohn&#8217; s disease.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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