Pregled bibliografske jedinice broj: 154346
Cytokinins in fertilised flowers of Helleborus niger L.
Cytokinins in fertilised flowers of Helleborus niger L. // Conference Handbook, IPGSA Conference 2004: 18th International Conference on Plant Growth Substances / Medley, Elizabeth ; Cunningham, Suzy (ur.).
Canberra: International Plant Growth Substances Association, 2004. str. 110-110 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 154346 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Cytokinins in fertilised flowers of Helleborus niger L.
Autori
Salopek-Sondi, Branka ; Tarkowski, Petr ; Tarkowská, Dana ; Strnad, Miroslav ; Magnus, Volker
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Conference Handbook, IPGSA Conference 2004: 18th International Conference on Plant Growth Substances
/ Medley, Elizabeth ; Cunningham, Suzy - Canberra : International Plant Growth Substances Association, 2004, 110-110
Skup
IPGSA Conference 2004: 18th International Conference on Plant Growth Substances
Mjesto i datum
Canberra, Australija, 20.09.2004. - 24.09.2004
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Helleborus niger; Christmas rose; cytokinin; flower and fruit development
Sažetak
The (initially white) perianth of fertilized Christmas rose (Helleborus niger L.) flowers turns green and persists until the seeds are ripe. This process stops when the developing fruit are removed, but is restored by applying cytokinins in lanolin (Salopek-Sondi et al. : J. Plant Physiol. 157, 357, 2000 ; J. Exp. Bot. 53, 1949, 2002), thus suggesting a role for cytokinins in the metamorphosis of fruit-bearing flowers. LC-MS/MS analysis confirmed their presence in both the fruit and the perianth. We identified N^6-isopentenyladenine, zeatin, and dihyrozeatin bases as well as the corresponding 9-ribosides by tandem mass spectroscopy. In order to obtain satisfactory spectral information, the partially purified plant extracts were derivatized before LC-MS/MS. In a separate approach, fractionation by ion exchange and immunoaffinity chromatographies, combined with phosphatase and glucosidase treatments and LC-MS, indicated the additional presence of citokinin ribotides and glucosides in significant quantities. The abundance of cytokinins (in particular, trans-zeatin and derivatives) in the fruit increased enormously during early seed development and remained high until about 2 weeks before fruit ripening. The steep rise in fruit cytokinins coincided with the greening process in the perianth, but the changes in cytokinin levels in the perianth itself were less pronounced.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
