Naslov
Immunolocalization of vacuolar-type H+-ATPase in rat submandibular gland and adaptive changes induced by acid-base disturbances

Autori
Roussa, Eleni ; Thevenod, Frank ; Sabolić, Ivan ; Herak Kramberger, Carol Mirna ; Nastainczyk, Wolfgang ; Bock, Rudolf ; Schulz, Irene

Izvornik
The journal of histochemistry and cytochemistry (0022-1554) 46 (1998), 1; 91-100

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
salivary gland; proton pump; immunocytochemistry; ion transport
(salivary gland; proton pump; immunocytochemisty; ion transport)

Sažetak
Using antibodies against the 31-kD and 70-kD subunits of vacuolar type H+-ATPase (V-ATPase)and light microscopic immunocytochemistry, we have demonstrated the presence of this V-ATPase in rat submandibular gland. We have also investigated the adaptive changes of this transporter during acid-base disturbances such as acute and chronic metabolic acidosis or alkalosis. Our results show intracellularly distributed V-ATPase in striated, granular, and main excretory duct cells in controls, but no V-ATPase immunoreaction in acinar cells. Both acute and chronic metabolic acidosis caused a shift in V-ATPase away from diffuse distribution towards apical localization in striated and granular duct cells, suggesting that a V-ATPase could be invovlved in the regulation of acid-base homeostasis.In contrast, during acidosis the main excretory duct cells showed no changes in the V-ATPase distribution compared to controls. With acute and chronic metabolic alkalosis, no changes in the V-ATPase distribution occured.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA