Naslov
Molecular cloning and chimerisation of CDI 315B monoclonal antibody

Autori
Kopitar-Jerala, Nataša ; Bastagno, Marco ; Fan, Xiaohui ; Novak Despot, Đurđica ; Burrone, Osear ; Kos, Janko ; Škrk, Janez ; Gubenšek, Franc

Izvornik
Pflügers Archiv (0031-6768) 439 (2000); R79-R80

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
breast cancer; recombinant antibodies; chimerisation

Sažetak
A chimeric mouse-human antibody has been created that recognizes an antigen found on breast cancer cells and melanoma cells. Immunoglobulin constant domains of mouse monoclonal antibody CDI 315B Cgl and Ck, were substituted by the human Cgl and Ck. The CDI 315B variable heavy and light chain regions were PCR amplified from hybridoma RNA and sequenced. Mouse variable Vh and Vl regions were joint to human IgG1 and k constant regions and subcloned into pcDNA3 expression vectors. The Sp2/0 murine myeloma cells were transfected with expression vectors pcDNA3L and pcDNA3H and the reactivity of chimeric antibodies was tested by indirect ELISA using B16F1 murine melanoma cells as well as MCF7 human cancer cells, as antigen.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA