Naslov
Resonance Raman Study on Pentacoordinated Ferrous Nitrosyl Myoglobin - The Influence of pH

Autori
Mackin, Helen C. ; Benko, Bojan ; Yu, Nai-Teng ; Gersonde, Klaus

Izvornik
FEBS letters (0014-5793) 158 (1983), 2; 199-202

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Electron spin resonance ; Isotope shift ; Myoglobin ; Nitrosyl myoglobin ; Resonance Raman

Sažetak
The resonance Raman (RR) spectra of the hexacoordinated ferrous nitrosyl sperm whale myoglobin are independent of pH for both the 200-700 cm and 1350-1650 cm-1 regions. In agreement with earlier ESR [Eur. J. Biochem. (1972) 31, 578-584] and contrary to recent RR [Biochemistry (1982) 21, 6989-6995] results the RR spectra do not indicate a transition to the pentacoordinated ferrous nitrosyl derivative at low pH. However, interaction of the protein with sodium dodecylsulfate, leads to the formation of a pure pentacoordinated state with a typical RR spectrum. Replacement of 14NO for 15NO in this pentacoordinated ferrous nitrosyl derivative does not exhibit isotope effects using 413.1 excitation.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

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