Naslov
Dictyostelium discoideum: a model organism on the eve of the genome sequencing completion

Autori
Barišić, Karmela ; Weber, Igor

Izvornik
Periodicum biologorum (0031-5362) 106 (2004); 103-114

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Dictyostelium discoideum; genome project; model organism; endocytosis; cell motility; cytokinesis; multicellular development; molecular genetics

Sažetak
The social amoeba Dictyostelium discoideum is a well-established model organism for the study of basic aspects of differentiation, signal transduction, phagocytosis, cytokinesis and cell motility. The molecular genetic techniques available include gene inactivation by homologous recombination, gene replacement, antisense strategies, restriction enzyme-mediated integration (REMI), RNA interference (RNAi), library complementation and expression of proteins fused to green fluorescent protein (GFP). International efforts are underway that aim at determining the complete Dictyostelium genome sequence. The genome sequence, together with powerful molecular genetic tools, will undoubtedly further accelerate Dictyostelium research into a number of fundamental biological processes that are common to a wide range of eukaryotes. Furthermore, it will constitute the basis for genome-wide functional analyses. The integration of results from these studies should ultimately lead to a better understanding of the complex networks that govern cellular behaviour and development.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA