Pregled bibliografske jedinice broj: 152153
Disruption of Mre 11 function in Arabidopsis leads to a stochastic loss of telomeric DNA and chromosome end-to-end fusions at a fractions of telomeres
Disruption of Mre 11 function in Arabidopsis leads to a stochastic loss of telomeric DNA and chromosome end-to-end fusions at a fractions of telomeres // EMBO Workshop/58th Harden Conference : Telomeres and Genome Stability
Cambridge, Ujedinjeno Kraljevstvo, 2004. str. 13-13 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 152153 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Disruption of Mre 11 function in Arabidopsis leads to a stochastic loss of telomeric DNA and chromosome end-to-end fusions at a fractions of telomeres
Autori
Puizina, J. ; Siroky, J. ; Heacock, M. ; Mokros, P. ; Schweizer, D. ; Shippen, D. ; Vyskot, B. ; Riha, K.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
EMBO Workshop/58th Harden Conference : Telomeres and Genome Stability
/ - , 2004, 13-13
Skup
EMBO Workshop/58th Harden Conference: Telomeres and Genome Stability
Mjesto i datum
Cambridge, Ujedinjeno Kraljevstvo, 03.04.2004. - 07.04.2004
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Mre11; telomeric DNA; chromosome end-to-end fusions; Arabidopsis
Sažetak
We studied consequences of Mre11 dysfunction in an Arabidopsis line with a T-DNA insertion in the conserved N-terminal region of the AtMre11 gene. Plants homozygous for the insertion are viable but they exhibit severe growth defects. Cytogenetic analyses of mitotic sperads revealed abundant dicentric chromosomes and chromosome fragments. The frequency of chromosome fusions did not decrease in mre11 ku70 double mutants suggesting that plants possess robust DNA end-joining acticities that are independent of Ku and Mre11 complexes. The presence of subtelomeric sequences at a portion of anaphase bridges, detected by FISH, indicated that these bridges may result from chromosome fusions. The presence of end-to-end chromosome fusions in mre11 mutants was further confirmed by cloning telomere-telomere fusion junctions by PCR using primers specific for subtelomeric sequences. Interestingly, the average length of telomeres in mre11 mutants did not differ from that in the wild type control although the amount of telomeric DNA remaining at the telomere-telomere fusion junctions was substantially lower than the shortest detected telomeres. These data indicate that the genome instabilities observed in Mre11-deficient cells are, at least in part, derived from a stochastic loss of telomere function at a subset of chromosome termini.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
0177143
Ustanove:
Prirodoslovno-matematički fakultet, Split
Profili:
Jasna Puizina
(autor)
