Naslov
Effects of cortisole on inhibitory effect of halothane on human tumour cells, in vitro study

Autori
Kvolik, Slavica ; Glavaš-Obrovac, Ljubica ; Karner, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 11 European Society of Anaesthesiologists (ESA) Anniversary Meeting and 25th EAA Annual Meeting Euroanaesthesia 2003 ; u: European Journal of Anaesthesiology. Supplement 20 (2003) (S30) ; A-482 / Adams, A.P. - London : Delhi : Greenwich Medical Media, 2003

Skup
ESA Anniversary Meeting (11 ; 2003) ; EAA Annual Meeting Euroanaesthesia (25 ; 2003)

Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 12.04.2003. - 14.04.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cortisol; inhaled anaesthetics; antitumour effects

Sažetak
The aim of this in vitro assay is to evaluate whether addition of cortisole in dose observed intra-operatively modifies cell growth inhibition achieved by inhaled anaesthetic halothane. Human breast carcinoma (Zr-75, SKBr), human large bowel carcinoma (CaCo2) cells and normal fibroblasts (WI38) with cortisole 3μ g ml-1 were exposed to halothane 1.5 vol. in O2: N2O 35:60% and CO2 5% respectively. Exposition was performed 3 times at 37 C for 6 hours. Cytotoxicity of anaesthetics was analysed by using of validated tetrazolium dye assay (MTT): Cells were incubated at 37 C, after 24 hours 20 L of 5 mg/ml MTT in phosphate buffered saline was added to each well. Medium was removed after 4 hours and cells were washed out. The plates were transferred to Elisa reader (Stat fax 2100) to measure the dye extracted at 570 nm. Three groups of cells were analysed: exposed to halothane, to cortisole and halothane and incubated with cortisole alone. Differences of 20% and more were assumed as significant. Results observed in assay are shown on fig 1. There was not found a signifficant differece between cell lines exposed to halothane and cortisole, and halothane alone (correl = 0.9716). Addition of cortisole does not modify inhibitory effects of halothane to tumour cells growth. Reference Elena G, Puig NR. Bay ML. et al. Int J Immunopharmacol. 1997 ; 19:699-707.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

Napomena
Rad je kap predavanje prezentiran i na skupu 10th European Society of Anaesthesiologists (ESA) Anniversary Meeting and 24th ESA Annual Meeting Euroanaesthesia 2002, održanom od 06.09.2002., Nica, Francuska ; sažetak je objavljen u European Journal of Anaesthesiology. Supplement 19 (2002) (S24) / A.P. Adams (ur.). ; 2002. 114-114.

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