Naslov
Synergistic effects of transforming growth factor beta and interleukin 1 alpha on prostaglandin E2 synthesis in bone cells

Autori
Marušić, Ana ; Kalinowski, Judith ; Harrison, John ; Centrella, Michael ; Lorenzo, Joseph

Izvornik
Journal of bone and mineral research (0884-0431) 5 (1990), 2; 12-12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
interleukin 1alpha ; transforming growth factor beta

Sažetak
Both interleukin 1alpha (IL-1) and transforming growth factor beta (TGF beta) stimulate prostaglandin synthesis in osteoblasts. We investigated the effects of a combination of these two agents on PGE2 production in the murine osteoblast-like cell line, MC3T3-E1 and primary rat osteoblats-like cells. IN DMEM with 10% FCS, IL-1 was a potent stimulator of PGE2 production (50-fold increase in PGE2 at 0.1ng/ml IL-1 measured by RIA). TGF beta (up to 10ng/ml) had only a small effect alone and no additional effect on IL-1 responses. In serum-free conditions, cell responses were markedly altered.IL-1 alone (0.1- 100ng/ml) had little or no effect on PGE2 production. However, simultaneous addition of TGF beta restored the ability of IL-1 to increase PGE2 production (10- fold stimulation with 0.1ng/ml of both IL-1 and TGFbeta). This effect was seen as early as 6h after the addition of both agents.As with MC3T3-E1 cells, serum free primary osteoblast cultures did not show PGE2 response to IL-1 alone (0.1-10ng/ml) but did respond with 10ng/ml TGF beta (6-fold increase over TGF beta alone). The effect of TBF beta on IL-1 responses in the MC3T3- E1 cells was not associated with changes in IL-1 alpha binding (measured by Scatchard analysis) or steady-state levels of PGH synthase (cyclooxygenase) mRNA levels (measured by Northern blot analysis). These results demonstrate that in serum-free cultures of osteoblast-like cells, IL-1 and TGF beta synergistically interact to stimulate PGE2 synthesis. This interaction may be important for normal bone turnover and the responses of bone to inflammatory stimuli.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA