Naslov
Toxigenic potency of Fusarium species isolated from non-harvested maize

Autori
Cvetnić, Zdenka ; Pepeljnjak, Stjepan ; Šegvić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CROTOX 2004 / - Zagreb, 2004

Skup
3rd Croatian Congress of Toxicology with International Participation

Mjesto i datum
Plitvička Jezera, Hrvatska, 26.05.2004. - 29.05.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Fusarium; trichotecenes; fumonisin B1; zearalenone

Sažetak
The most frequent "field fungi" are Fusarium species, which can colonise the maize before harvest causing spoilage, and production of mycotoxins. Members of Fusarium genera are potent producers of trichotecens (DON, DAS, T-2), zearalenone and fumonisin B1. These mycotoxins are associated with various animal and human toxicoses and/or carcinomas. The aim of the study was to determine the mycoflora content and toxigenic potential of Fusarium species isolated from maize left in field over winter in Croatia. A total of 112 maize samples were collected over a two years period (in 1999 and in 2003). Mycological analyses of maize seeds were done using Sabouraud agar with antibiotics and sterile humid filter paper plates. Fusarium species were identified on basis of morphological macroscopic and microscopic characteristic on Potato-dextrose and Synthetic nutrient agar. Production of trichotecens (DON, DAS, T-2), zearalenone and fumonisin B1 was performed in biosynthesis on liquid yeast medium for 10 days at 25 20C. Isolation and detection of trichotecens and zearalenone were performed by multytoxin extraction and TLC method and modified method for detection of fumonisin B1. Fusarium species were the most frequent fungi in maize founded with 78, 6% in 1999 and 85, 0% in 2003. Among fusaria F. verticillioides was dominant species founded in 12, 5% (1999) and 35, 7% (2003) maize samples. Other determined fusaria were F. graminearum (8, 9% in 2003), F. poae and F. sporotrichoides (2, 0-3, 6%), F. tricinctum and F. tabacinum (2, 0% in 1999). Production of FB1 was established for all F. verticillioides isolated in 1999 in concentration range 280-918 mg/L, and for 55% of strains founded in 2003 (48-400 mg/L). Three strains also produced zearalenone: 1 strain in 1999 produced 3, 80 mg/L and 2 strains in 2003 produced 20, 0-70, 0 mg/L. In addition 4 strains of F. graminearum isolated in 2003 produced higher amounts of zearalenone (60, 0-180, 0 mg/L). T-2 production ability was found in F. tricinctum (1, 55 mg/L) isolated in 1999. Higher production amounts of zearalenone founded in strains isolated in 2003 can be due to environmental conditions especially seasonal temperature oscillation, which was significant during this sampling period.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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