Naslov
Distribution and fumonisin B1 production capacity of Fusarium moniliforme isolated from corn in Croatia

Autori
Šegvić, Maja ; Pepeljnjak, Stjepan

Izvornik
Periodicum biologorum (0031-5362) 105 (2003), 3; 275-279

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Fusarium moniliforme; Fumonisin B1; Corn contamination; Endemic nephropathy

Sažetak
Background and Purpose: Fusarium moniliforme, a producer of fumonisin B1 (FB1), is a common contaminant to corn cultivations in all parts of the world, and it has been associated with several diseases in animals and humans. Since FB1 showed a nephrotoxic effects in different sorts of animals, it may bee involved in ethiology of endemic nephropathy (EN) in Croatia (Slavonski Brod). The purpose of our study was to determine distribution and FB1 production capacity of F. moniliforme isolated from non-harvested and stored (domestic households) corn collected from several parts of Croatia (Karlovac, Sisak, Daruvar, Slavonski Brod, Osijek, Vinkovci). Materials and Methods: Samples of non-harvested (N=136) and stored (N=136) corn were mycologically analysed by using Sabouraud agar and sterile humid filter paper plates. Fusarium moniliforme strains were identified on basis of morphological macroscopic and microscopic characteristic on Potato-dextrose agar. Fumonisn B1 production was performed in biosynthesis on liquid yeast medium for 10 days at 25 20C. Isolation and detection of FB1 were performed by modified multytoxin extraction and TLC method on silica gel GF254 in mobile phase acetonitrile/toluene/water (95:5:2), with standard solution of FB1 (0.5 mg/mL), as comparable substance. Detection of FB1 was performed under the UV light (366 nm). Results: In total number of corn samples (non-harvested and stored) Fusarium was dominated genera (86% and 56% respectively). Production of FB1 was determined in 100% F. moniliforme strains in concentration range 181-1320 mg/L. Producer strains were ranged in three groups based on fumonisinotoxinogenicity rate, as high (881-1320 mg/L), intermediate (441-880 mg/L) and low (181-440 mg/L) FB1 producers. Highest number of high and intermediate producers was found in corn from eastern part of Croatia (Vinkovci and Slavonski Brod) (23-54% and 5-35 % respectivelly). Conclusions: High percent of occurrence of F. moniliforme in corn and high production capacity of strains examined, particularly those from Endemic nephropathy area (Slavonski Brod), point on potential health hazard for animals and humans.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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