Naslov
Comparison of advanced glycation endproducts on haemoglobin (Hb-AGE) and haemoglobin A1c for the assessment of diabetic control

Autori
Turk, Zdenka ; Mesić, Ranko ; Benko, Bojan

Izvornik
Clinica chimica acta (0009-8981) 277 (1998), 2; 159-170

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diabetes mellitus; advanced glycation endproducts; glycated haemoglobin

Sažetak
Glycation process in vivo results in two different products: early and advanced glycation endproducts (AGEs). The mechanism of early product formation has been well described, with HbA(1c) as the best-studied example. The finding that advanced glycation endproducts are also formed on haemoglobin suggests that HbA(1c) is a precursor for Hb-AGE formation. HbA(1c) has been well established as an important indicator for glycaemia monitoring, but the diagnostic role ;of Hb-AGE has not yet been clarified. A question is whether HbA(1c) and Hb-AGE are competitive or complementary parameters. In our study, Hb-AGE was quantified by the competitive ELISA technique using polyclonal anti-AGE-RNase antibodies to detect AGE immunoreactivities of proteins precipitated in red cell hemolysate. Results are expressed as AGE units/mg Hb. Hb-AGE was analysed in three groups of patients divided according to HbA(1c) values as follows: group I (n = 25) HbA(1c) < 7%, Hb-AGE = 6.93 (5.7-7.3) U/mg; group II (n = 25) HbA(1c) = 7-10%, Hb-AGE = 8.62 (7.7-10.2) U/mg; and group III (n = 25) HbA(1c) >10%, Hb-AGE = 12.47 (10.8-13.9) U/mg (median (interquartile range)). A close relation between the amounts of red cell HbA(1c) and Hb-AGE was observed in all diabetic subjects (n = 75) r = 0.77, P < 0.001. Patients with HbA(1c) level >8% were considered to be in poor glycaemic control and those with HbA(1c)

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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