Pregled bibliografske jedinice broj: 149685
Radioimmunoassay quantification of intra- and extracellular interleukin 1 alpha in murine macrophages, osteoblastic cell line and calvarial cultures
Radioimmunoassay quantification of intra- and extracellular interleukin 1 alpha in murine macrophages, osteoblastic cell line and calvarial cultures // Croatian medical journal, 32 (1992), 207-212 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 149685 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Radioimmunoassay quantification of intra- and extracellular interleukin 1 alpha in murine macrophages, osteoblastic cell line and calvarial cultures
Autori
Marušić, Ana ; Jastrzebski, Sandra ; Kalinowski, Judith ; Lorenzo, Joseph
Izvornik
Croatian medical journal (0353-9504) 32
(1992);
207-212
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Bone cells ; Interleukin 1 ; Macrophage ; Radioimmunoassay
Sažetak
Interleukin 1 alpha and beta are two forms of interleukin 1 (IL-1) produced by a variety of activated cells, primarily by monocytes and macrophages.The development of specific sera and monoclonal antibodies made it possible to measure the forms of IL-1 with the use of specific radioimmuno (RIA) and enzyme-linked immunosorbent (ELISA) assays.In this report, we describe a construction of a simple RIA for mouse IL-1 alpha sensitive down to 50 pg/ml of IL-1 alpha.The assay was used to quantify the extracellular (EC) and intracellular-cell associated (IC) IL-1 alpha in murine macrophage cell line P388D1, spleen macrophages, calvarial tissue cultures and osteoblastic cell line MC3T3-E1.IL-1 alpha could not be detected in supernatants from unstimulated P388D1 and spleen cells.In contrast, there was 7- to 12-fold more IC IL-1 alpha in P388D1 cells.LPS increased the IC level of IL-1 alpha at a concentration as low as 1 ng/ml.Maximal increase occurred after g hours of lipopolysaccharide (LPS) stimulation.Immunoprecipitation of the freeze-thawed P388D1 cell extracts produced a single 17kD band that competed away with cold murine IL-1 alpha.IL-1 alpha could not be detected in conditioned media from calvarial cultures even after 10-fold concentration.However, LPS induced a dose-dependent increase of IL-1 alpha in calvarial freeze-thawed extarcts.Neither unstimulated nor LPS-stimulated MC3T3-E1 cells, amurine non-transformed cell line with osteoblastic phenotype, produced any detectable EC or IC IL-1 alpha.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
Citiraj ovu publikaciju:
Časopis indeksira:
- Scopus
