Naslov
Efficiency of RAPD markers for varietal identification in olive (Olea europea L.) germplasm collections

Autori
Belaj, Angjelina ; Šatović, Zlatko ; Rallo, Luis ; Trujillo, Isabel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the EUCARPIA Symposium on Fruit Breeding and Genetics / Laurens, Francois - Angers : INRA Centre d'Angers, 2003, 93-93

Skup
EUCARPIA Symposium on Fruit Breeding and Genetics

Mjesto i datum
Angers, Francuska, 01.09.2003. - 05.09.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Olea europea; RAPDs; variety; germplasm

Sažetak
The aim of this study was to evaluate the resolivng power of RAPD markers for rapid and reliable identification of olive cultivars in germplasm collections. The D parameter (the probability that two randomly chosen cultivars have different banding patterns), used for that purpose, showed high values for most of the 21 primers used and its values ranged from 0.6114 (OPI-13) to 0.9762 (OPK-16) with a mean value of 0.8566. This parameter was used to select the five most discriminating primers: OPK-16, OPA-19, OPX-09, OPF-O6 and OPZ-11. The joint confusion probability and the statistical number of undistinguishable pairs of cultivars were estimated for these primers (under independence hypothesis). The combiantion of three primers (OPK-16, OPA-19 and OPX-09) was found optimal for rapid discrimination of 103 cultivars with a very low value of cumulative confusion probability (1.72 x 10-5), leaving 0.0903 pairs of cultivars undistinguishable. This fact together with the efficiency of the most discriminating primers combination on an increasing number of cultivars evidenced the utility of RAPD markers for identification of olive cultivars in collections and in nurseries.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA