Naslov
Detection of stress-70 protein isoforms in marine mussel Mytilus galloprovincialis using 2-D gel electrophoresis

Autori
Hamer, Bojan ; Muller, E.G. Werner ; Batel, Renato

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
3rd Croatian Congress of Toxicology - CROTOX 2004

Mjesto i datum
NP Plitvička jezera, Hrvatska, 26.05.2004. - 29.05.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Stress-70 proteins; HSP70; HSP72; Mytilus galloprovincialis; 2-D gel electrophoresis

Sažetak
In the present work we investigated isoforms of stress-70 proteins in the gills of mussel M. galloprovinicialis using 2-D gel electrophoresis. 1-D analysis (SDS-PAGE) using monoclonal mouse antibodies anti-HSP70 detected two bands of stress-70 proteins 70 and 72 kDa. 2-D analysis (IEF + SDS-PAGE + WB) proved that used antibodies detected HSP70 (pI 5.7-5.9) and HSP72 (pI 5.5-5.6). Image analysis of 2-D gels stained with GelCode Blue (commasie) and corresponding Western Blot were done using gel comparison program Flicker (http://www.lecb.ncifcrf.gov/flicker/) which runs on any www connected computer and is invoked from a Java-capable browser. Results indicated that it is possible detection and quantification of stress-70 protein isoforms on 2-D stained gels without WB analysis. The usefulness and problems of such detection of stress-70 proteins as biomarkers of environmental pollution is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija

POVEZANOST RADA