Naslov
Grapevine Yellows-spread of the disease in Croatia

Autori
Ćurković Perica, Mirna ; Šeruga, Martina ; Kozina, Bernard ; Krajačić, Mladen ; Škorić, Dijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Extended abstracts of the 14th meeting of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine / Boscia, Donato - Locorotondo : Department of Plant Protection and Applied Microbiology, University of Bari, 2003, 95-95

Skup
14th meeting of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine

Mjesto i datum
Bari, Italija; Locorotondo, Italija, 12.09.2003. - 17.09.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
phytoplasmas; 16S rRNA gene; grapevine yellows; geographical distribution

Sažetak
Grapevine yellows diseases (GY) are mainly caused by plant pathogenic prokaryotes – phytoplasmas. Since 1994, when symptoms typical for GY were observed in Zagreb region, spreading of the phytoplasmoses was monitored throughout the country. Up to date, GY symptoms were observed in all parts of the country except on the islands. In north-western Croatia the spread of the phytoplasmoses was wide, while in the eastern part it even had an epidemic character, causing significant economic losses. In Ilok and Baranja up to 50% of the vines were infected. The most susceptible cultivar is Chardonnay, followed by Pinot Gris, Pinot Noir, Pinot Blanc, Ugni Blanc and Cabernet Sauvignon. Infection was also confirmed in indigenous cultivars: Debit, Plavina, Maraština and Istarska Malvazija. The detection of the pathogen in different grapevine samples collected all over the country was based on nested PCR (polymerase chain reaction) that employed phytoplasma generic and/or specific primers amplifying 16S rRNA gene. PCR conditions were as previously described by Šeruga et al. The RFLP (restriction fragment length polymorphism) with a set of restriction enzymes enabled classification of the phytoplasmas from all grapevine samples in the same ribosomal subgroup 16SrXII-A.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA