Naslov
Suppression of cytological defects in recBC sbcBC ruv mutants of Escherichia coli

Autori
Zahradka, Davor ; Zahradka, Ksenija ; Petranović, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EMBO Conference on Molecular Microbiology: Exploring Prokaryotic Diversity - Abstract Book / De Lorenzo, Victor ; Toussaint, Ariane ; Bernander, Rolf - Heidelberg : EMBL, 2004, 127-127

Skup
EMBO Conference on Molecular Microbiology: Exploring Prokaryotic Diversity

Mjesto i datum
Heidelberg, Njemačka, 22.04.2004. - 26.04.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Escherichia coli; recBC sbcBC; RuvABC proteins; Holliday junctions; chromosome segregation; cell division

Sažetak
The RuvABC proteins of Escherichia coli play an important role in the processing of Holliday junctions during homologous genetic recombination. We have found that a ruvABC deletion severely impairs cell growth, chromosome segregation, and cell division in exponentially growing recBC sbcBC cells. We showed that recA and recF mutations significantly improved chromosome partitioning and cell division but not viability of recBC sbcBC ruv cells. All ruv-associated cytological defects were suppressed by the rus-1 mutation which is known to induce synthesis of an alternative Holliday junction resolvase, the RusA protein. Our results suggest that in recBC sbcBC ruv mutants abortive recombination takes place during exponential growth. The unresolved Holliday junctions accumulated during this process cause cytological defects and affect cell viability.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA