Naslov
Yeast non-replicative plasmids and strain construction

Autori
Zgaga, Zoran ; Koren, Predrag ; Ninković, Milena ; Gjuračić, Krešimir ; Alačević, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
VIth European Congress on Biotechnology, Book of abstracts / - Firenza : München, 1993

Skup
VIth European Congress on Biotechnology

Mjesto i datum
Firenca, Italija, 13.06.1993. - 17.06.1993

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
yeast; transformation; non-replicative plasmids; gene conversion

Sažetak
Stable transformation with yeast non-replicative vectors relies on homologous recombination between yeast chromosome and plasmid molecule. We show that UV-irradiation of double-stranded plasmids stimulates this process. Irradiation of either double or single-stranded non-replicative plasmids does not lead to detectable mutagenesis. Transformation by conversion of point mutations is favored if single-stranded plasmids are UV-irradiated. Linearized single-stranded plasmids may even convert the insertions of the Ty element (6.1 kb).

Izvorni jezik
Engleski

POVEZANOST RADA