Naslov
Illegitimate integration of single-stranded non-replicative plasmids in Saccharomyces cerevisiae chromosomes

Autori
Gjuračić, Krešimir ; Zgaga, Zoran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of Croatian Biochemists HB94, Book of abstracts / - Opatija, 1994, 71-P46

Skup
Annual meeting of Croatian Biochemists HB94

Mjesto i datum
Opatija, Hrvatska, 14.10.1994. - 15.10.1994

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
yeast; illegitimate recombination; single-stranded DNA

Sažetak
Yeast transformation with non-replicative plasmids is considered to be mediated by recombination with homologous chromosomal sequences. However, we have found that the linearized single-stranded (ss) plasmids transform yeast cells mainly by illegitimate integration even in the presence of extended homology with the yeast genome. Integration of plasmid DNA occurs at different chromosomal loci and is very often associated with multiple and /or complex plasmid integrations. To further investigate the ability of ss plasmids to transform yeast cells via illegitimate integration, we developed an experimental system where transformation is performed in complete absence of homology between plasmid DNA and yeast genome. We used circular and linearized plasmids in either single- or double-stranded form. We found out that both linearized and circular ss plasmids transform yeast cells with approximately 100-fold higher efficiencies than their double-stranded counterparts. Possible role of single-stranded DNA in generation of genetic variability will be discussed.

Izvorni jezik
Engleski

POVEZANOST RADA