Pregled bibliografske jedinice broj: 147366
Yeast non-replicative plasmids and strain construction
Yeast non-replicative plasmids and strain construction // Proceedings of the VIth European Congress on Biotechnology / Alberghina, L ; Frontali, L ; Sensi, P (ur.).
New York (NY): Elsevier, 1994. str. 327-330 (poster, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
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Naslov
Yeast non-replicative plasmids and strain construction
Autori
Zgaga, Zoran ; Koren, Predrag ; Ninković, Milena ; Gjuračić, Krešimir ; Alačević, Marija
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Proceedings of the VIth European Congress on Biotechnology
/ Alberghina, L ; Frontali, L ; Sensi, P - New York (NY) : Elsevier, 1994, 327-330
Skup
VIth European Congress on Biotechnology
Mjesto i datum
Firenca, Italija, 13.06.1993. - 17.06.1993
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
yeast; transformation; non-replicative plasmids; gene conversion
Sažetak
Stable transformation with yeast non-replicative vectors relies on homologous recombination between yeast chromosome and plasmid molecule. We show that UV-irradiation of double-stranded plasmids stimulates this process. Irradiation of either double or single-stranded non-replicative plasmids does not lead to detectable mutagenesis. Transformation by conversion of point mutations is favored if single-stranded plasmids are UV-irradiated. Linearized single-stranded plasmids may even convert the insertions of the Ty element (6.1 kb).
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Profili:
Krešimir Gjuračić
(autor)
Marija Alačević
(autor)
Milena Ninković
(autor)
Predrag Koren
(autor)
Zoran Zgaga
(autor)