Naslov
Mutational Analysis of p53 in Human Tumors. Direct DNA Sequencing and SSCP
(Mutational analysis of p53 in human tumors. Direct DNA sequencing and SSCP)

Autori
Erster, Susan ; Slade, Neda ; Moll, Ute M

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Methods in Molecular Biology: p53 Protocols

Urednik/ci
Deb, Sumitra ; Palit Deb, Swati

Izdavač
Humana Press

Grad
Totowa (NJ)

Godina
2003

Raspon stranica
219-230

ISBN
1-58829-106-5

Ključne riječi
p53, tumors, mutations, PCR, SSCP, DNA sequencing

Sažetak
This chapter describes the techniques necessary to identify p53 genomic mutations in either frozen of paraffin-embedded tumor samples. DNA is extracted from the tumor samples and then used as a template to amplify the p53 coding sequences by polymerase chain reaction (PCR). The PCR products are characterized by agarose gel electrophoresis. Subsequently, mutations can be detected by direct DNA sequencing of the PCR products, followed by acrylamide gel electrophoresis and autoradiography. Alternatively, PCR products containg mutations can be identified by their aberrant mobility on denaturing acrylamide gels, using single-stranded conformation polymorphism (SSCP).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA