Naslov
Activation of carbonic anhydrase in branchial cavity tissue of lobster (Homarus gammarus) by dilute seawater exposure

Autori
Pavičić-Hamer, Dijana ; Devescovi, Massimo ; Lucu, Čedomil

Izvornik
Journal of Experimental Marine Biology and Ecology (0022-0981) 287 (2003); 79-92

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Carbonic anhydrase; Dilute seawater; Branchial cavity tissues; lobster; Homarus gammarus

Sažetak
Activitiy of carbonic anhydrase and its distribution in the branchial cavity tissues were studied in European lobster Homarus gammarus from ambient seawater (salinity = 38 ppt, 1126 mosmol/l ) and acclimated to dilute seawater (salinity=20 ppt ; 548 mOsmol/l). Acetazolamide inhibited dose dependently the activity of carbonic anhydrase in homogenates of epipodites, where the inhibition constant (IC50=0.12 microM) did not differ significantly from that of membrane vesicles and cytosolic fraction. In subcellular fraction from DSW -acclimated lobsters, the main proportion of 75.8% (epipodite), 61.0% (branchiostegite) of total carbonic anhydrase in each of these tissues remained in cytosol portion. Partially purified membranes contained 6.8% (epipodite) and 16.2% (branchiostegite) and the remainder of 15% (epipodite) and 27% (branchiostegite) was found in mitochondrial and nuclear fractions. In gills, 49.2% and 9% of total gill carbonic anhydrase activity was found respectively in cytosol and partially purified vesicles and the rest in mitochondrial and nuclear fractions.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

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