Naslov
Hepatic and intestinal alkaline phosphatase activity in murine obstructive jaundice

Autori
Varljen, Jadranka ; Milin, Čedomila ; Eraković, Vesna ; Ravlić-Gulan, Jagoda ; Petković, Marija ; Ćuk, Mira ; Dvornik, Štefica ; Radošević-Stašić, Biserka ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum Biologorum 100 (1998), suppl. 3 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1998, 76-76

Skup
Fourth international meeting "Mechanisms in local immunity" and Fourth meeting of Alps Adria society for immunology of reproduction

Mjesto i datum
Opatija, Hrvatska, 16.09.1998. - 19.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PGM; cholestasis; intestinal alkaline phosphatase activity

Sažetak
Biliary obstruction causes a significant immune dysfunction, which may be responsible for serious postoperative complications and high mortality rate. The effects might be linked with liver injury and with biochemical alterations in jaundiced serum, but the pathogenetic mechanisms are still unclear. Owing to the importance of gut-associated immunity for the proper functioning of immune system, in this work we attempted to investigate the changes of hepatic and intestinal alkaline phosphatase activity (ALP) in cholestatic mice, since it is well known that this enzyme well correlate with the functional state of mucosal and hepatic tissue. Experimental jaundice was induced by common bile duct ligation in mice which were sacrificed 1, 2, 3 or 7 days later. The ALP activity was determined by method of Mc Comb at al. in samples of liver and six different location of mucosa in gastrointestinal tract (antrum, duodenum, jejunum, ileum, colon and rectum). The data have shown that ALP activity in the liver was rising proportionally with the duration of the jaundice. ALP activity in gastrointestinal tract (GIT), however, was differentially affected with various locus-dependent kinetics. In initial phase a significant fall of ALP activity was observed in duodenum (on 1st and 2nd day) and in rectum (2nd day), while in the later phase the significant increase of ALP activity was seen in ileum and colon. The data suggest that during the cholestatic jaundice occurs not only the damage in the liver, but also on the level of GIT mucosa. It could be hypothesized that the latter, altering the function of local lymphatic system and permitting the bacterial translocation into portal system, might result in endotoxinemia, which contributes to the dysfunction of immune system, seen in icterus.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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