Naslov
Influence of indole compounds on cellular level of glutathione

Autori
Durgo, Ksenija ; Jakopec, Sanjica ; Franekić-Čolić, Jasna ; Osmak, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2. Znanstveni simpozij s međunarodnim sudjelovanjem 45 godina molekularne biologije u Hrvatskoj 50 godina dvostruke uzvojnice Knjiga sažetaka / Ambriović Ristov , Andreja ; Brozović, Anamaria - Zagreb : Institut Ruđer Bošković, 2003, 42-42

Skup
2. Znanstveni simpozij s međunarodnim sudjelovanjem 45 godina molekularne biologije u Hrvatskoj 50 godina dvostruke

Mjesto i datum
Zagreb, Hrvatska, 20.11.2003. - 21.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
indole compounds; glutathione

Sažetak
Cruciferous vegetables such as cauliflower, cabbage, kale, kohlrabi, turnips or broccoli, contain mg/g levels of glucosinolate glucobrassicin (indolyl methyl glucosinolate). Indole-3-carbinol is the major hydrolytic product obtained from glucobrassicin. When the plant cells are damaged by cutting or chewing, a thioglucosidase-mediated autolytic process takes place generating indole-3-carbinol, glucose and thiocyanate ion. Recent reports have shown that indole compounds can modulate cellular response when cells are exposed to variety of environmental xenobiotics. In our previous work we have shown that indole-3-carbinol and cauliflower extract did not induce point mutations in Salmonella typhimurium TA98 and TA100. We have also shown that indole compounds have inhibited formation of point mutations when Salmonella typhimurim TA98 and TA100 were treated with two standard mutagens 4-nitroquinoline-1-oxide (direct acting mutagen) and 2-aminoanthracene (promutagen). In this work we have examined indole-3-carbinol, 3-indolylacetonitrile, indole-3-carboxaldehyde and cauliflower extract for their influence on cellular glutathione level in four cell lines: human laryngeal carcinoma cells (parental HEp2 and their cisplatin resistant subline CK2) and human cervix cancer (parental HeLa and their ciplatin resistant subline CK). We have measured and compared cellular concentration of glutathione after 72 hours of treatment of the cells with the highest nontoxic concentrations of indole-3-carbinol and cauliflower extract. Concentrations of glutathione in four cell lines were compared with the basal level of glutathione in untreated cells. It was shown that indole compounds have different influence on cellular level of glutathione ; indole-3-carboxaldehyde, indole-3-carbinol and cauliflower extract have cause increase of GSH level in Hep2 and HeLa cells. Indole-3-acetonitrile has caused increase of GSH level only in Hep2 cells. On the contrary, Hep CK2 cisplatin resistant cells have increased basal level of glutathione ; indole-3-carbinol and cauliflower extract decreased the level of glutathione, while indole-3-carboxaldehyde and indole-3acetonitrile didn’ t cause significant alteration of GSH level. In HeLa CK cell line, only cauliflower extract has caused slight increase of GSH level.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA