Pregled bibliografske jedinice broj: 142736
Acetylcholinesterase and butyrylcholinesterase activity measurements by the Ellman method: II. Comparison of the procedure protocol with procedures suggested by other authors
Acetylcholinesterase and butyrylcholinesterase activity measurements by the Ellman method: II. Comparison of the procedure protocol with procedures suggested by other authors // The Proceedings of the Third Chemical and Biological Medical Treatment Symposium (CBMTS III) / Price, Barbara ; Mack, Karla (ur.).
Aberdeen (MD): Applied Science and Analysis, 2003. str. 1-2 (poster, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 142736 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Acetylcholinesterase and butyrylcholinesterase activity measurements by the Ellman method: II. Comparison of the procedure protocol with procedures suggested by other authors
Autori
Reiner, Elsa ; Škrinjarić-Špoljar, Mira ; Šinko, Goran ; Simeon-Rudolf, Vera
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
The Proceedings of the Third Chemical and Biological Medical Treatment Symposium (CBMTS III)
/ Price, Barbara ; Mack, Karla - Aberdeen (MD) : Applied Science and Analysis, 2003, 1-2
Skup
The Third Chemical and Biological Medical Treatment Symposium (CBMTS III)
Mjesto i datum
Spiez, Švicarska, 05.05.2000. - 12.05.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Cholinesterase activity measurements; protocols
Sažetak
The procedure for plasma butyrylcholinesterase (BChE ; EC 3.1.1.8) activity measurement is well established, while this is not the case for the erythrocyte acetylcholinesterase (AChE ; EC 3.1.1.7). We suggest the separation of plasma from erythrocytes by centrifugation, followed by separate measurement (with 1.0 mM acetylthiocholine as substrate) of BChE in plasma and AChE in unwashed erythrocytes. The obtained results showed that the cholinesterase activity measured in whole blood equalled the summe of the AChE activity in suspended erythrocytes plus the BChE activity in plasma, calculated upon the measured haematocrit. In blood samples from 13 individuals, the summe of activities was 97 ± ; ; ; ; ; ; 7% of the activity measured in whole blood. The above procedure was compared with the method of Portmann (CBMTS, Dubrovnik 1998) based on activity measurements with acetylthiocholine and butyrylthiocholine which distinguish AChE from BChE, and with the method of Szinicz et al. (CBMTS, Dubrovnik 1998) where AChE activities are measured with acetylthiocholine in presence of ethopropazine, a BChE inhibitor. The advantage of our procedure is that both enzymes are measured with the same substrate and no inhibitor is required for the AChE and BChE assays. Further, the procedure can be performed with commercial kits for plasma activity measurements.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
0022014
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb
